Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases.
Identifieur interne : 000078 ( Main/Exploration ); précédent : 000077; suivant : 000079Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases.
Auteurs : Jennifer Millera Niem [Australie] ; Regina Billones-Baaijens [Australie] ; Benjamin Stodart [Australie] ; Sandra Savocchia [Australie]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2020.
Abstract
Grapevine trunk diseases (GTDs) are a serious problem of grapevines worldwide. The microbiota of the grapevine endosphere comprises prokaryotic and eukaryotic endophytes, which may form varied relationships with the host plant from symbiotic to pathogenic. To explore the interaction between grapevine endophytic bacteria and GTDs, the endomicrobiome associated with grapevine wood was characterized using next-generation Illumina sequencing. Wood samples were collected from grapevine trunks with and without external symptoms of GTD (cankers) from two vineyards in the Hunter Valley and Hilltops, NSW, Australia and metagenomic characterization of the endophytic community was conducted using the 16S rRNA gene (341F/806R) and ITS (1F/2R) sequences. Among the important GTD pathogens, Phaeomoniella, Phaeoacremonium, Diplodia and Cryptovalsa species were found to be abundant in both symptomatic and asymptomatic grapevines from both vineyards. Eutypa lata and Neofusicoccum parvum, two important GTD pathogens, were detected in low numbers in Hilltops and the Hunter Valley, respectively. Interestingly, Pseudomonas dominated the bacterial community in canker-free grapevine tissues in both locations, comprising 56-74% of the total bacterial population. In contrast, the Pseudomonas population in grapevines with cankers was significantly lower, representing 29 and 2% of the bacterial community in Hilltops and the Hunter Valley, respectively. The presence of Pseudomonas in healthy grapevine tissues indicates its ability to colonize and survive in the grapevine. The potential of Pseudomonas spp. as biocontrol agents against GTD pathogens was also explored. Dual culture tests with isolated fluorescent Pseudomonas against mycelial discs of nine Botryosphaeria dieback, three Eutypa dieback, and two Esca/Petri disease pathogens, revealed antagonistic activity for 10 Pseudomonas strains. These results suggest the potential of Pseudomonas species from grapevine wood to be used as biocontrol agents to manage certain GTD pathogens.
DOI: 10.3389/fmicb.2020.00477
PubMed: 32273871
PubMed Central: PMC7113392
Affiliations:
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University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Grapevine trunk diseases (GTDs) are a serious problem of grapevines worldwide. The microbiota of the grapevine endosphere comprises prokaryotic and eukaryotic endophytes, which may form varied relationships with the host plant from symbiotic to pathogenic. To explore the interaction between grapevine endophytic bacteria and GTDs, the endomicrobiome associated with grapevine wood was characterized using next-generation Illumina sequencing. Wood samples were collected from grapevine trunks with and without external symptoms of GTD (cankers) from two vineyards in the Hunter Valley and Hilltops, NSW, Australia and metagenomic characterization of the endophytic community was conducted using the 16S rRNA gene (341F/806R) and ITS (1F/2R) sequences. Among the important GTD pathogens, <i>Phaeomoniella, Phaeoacremonium, Diplodia</i> and <i>Cryptovalsa</i> species were found to be abundant in both symptomatic and asymptomatic grapevines from both vineyards. <i>Eutypa lata</i> and <i>Neofusicoccum parvum</i>, two important GTD pathogens, were detected in low numbers in Hilltops and the Hunter Valley, respectively. Interestingly, <i>Pseudomonas</i> dominated the bacterial community in canker-free grapevine tissues in both locations, comprising 56-74% of the total bacterial population. In contrast, the <i>Pseudomonas</i> population in grapevines with cankers was significantly lower, representing 29 and 2% of the bacterial community in Hilltops and the Hunter Valley, respectively. The presence of <i>Pseudomonas</i> in healthy grapevine tissues indicates its ability to colonize and survive in the grapevine. The potential of <i>Pseudomonas</i> spp. as biocontrol agents against GTD pathogens was also explored. Dual culture tests with isolated fluorescent <i>Pseudomonas</i> against mycelial discs of nine Botryosphaeria dieback, three Eutypa dieback, and two Esca/Petri disease pathogens, revealed antagonistic activity for 10 <i>Pseudomonas</i> strains. These results suggest the potential of <i>Pseudomonas</i> species from grapevine wood to be used as biocontrol agents to manage certain GTD pathogens.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32273871</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic <i>Pseudomonas</i> Against Grapevine Trunk Diseases.</ArticleTitle><Pagination><MedlinePgn>477</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2020.00477</ELocationID><Abstract><AbstractText>Grapevine trunk diseases (GTDs) are a serious problem of grapevines worldwide. The microbiota of the grapevine endosphere comprises prokaryotic and eukaryotic endophytes, which may form varied relationships with the host plant from symbiotic to pathogenic. To explore the interaction between grapevine endophytic bacteria and GTDs, the endomicrobiome associated with grapevine wood was characterized using next-generation Illumina sequencing. Wood samples were collected from grapevine trunks with and without external symptoms of GTD (cankers) from two vineyards in the Hunter Valley and Hilltops, NSW, Australia and metagenomic characterization of the endophytic community was conducted using the 16S rRNA gene (341F/806R) and ITS (1F/2R) sequences. Among the important GTD pathogens, <i>Phaeomoniella, Phaeoacremonium, Diplodia</i> and <i>Cryptovalsa</i> species were found to be abundant in both symptomatic and asymptomatic grapevines from both vineyards. <i>Eutypa lata</i> and <i>Neofusicoccum parvum</i>, two important GTD pathogens, were detected in low numbers in Hilltops and the Hunter Valley, respectively. Interestingly, <i>Pseudomonas</i> dominated the bacterial community in canker-free grapevine tissues in both locations, comprising 56-74% of the total bacterial population. In contrast, the <i>Pseudomonas</i> population in grapevines with cankers was significantly lower, representing 29 and 2% of the bacterial community in Hilltops and the Hunter Valley, respectively. The presence of <i>Pseudomonas</i> in healthy grapevine tissues indicates its ability to colonize and survive in the grapevine. The potential of <i>Pseudomonas</i> spp. as biocontrol agents against GTD pathogens was also explored. Dual culture tests with isolated fluorescent <i>Pseudomonas</i> against mycelial discs of nine Botryosphaeria dieback, three Eutypa dieback, and two Esca/Petri disease pathogens, revealed antagonistic activity for 10 <i>Pseudomonas</i> strains. These results suggest the potential of <i>Pseudomonas</i> species from grapevine wood to be used as biocontrol agents to manage certain GTD pathogens.</AbstractText><CopyrightInformation>Copyright © 2020 Niem, Billones-Baaijens, Stodart and Savocchia.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Niem</LastName><ForeName>Jennifer Millera</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Billones-Baaijens</LastName><ForeName>Regina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stodart</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Graham Centre for Agricultural Innovation (Charles Sturt University and NSW Department of Primary Industries), School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Savocchia</LastName><ForeName>Sandra</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Botryosphaeria dieback</Keyword><Keyword MajorTopicYN="N">Esca/Petri disease</Keyword><Keyword MajorTopicYN="N">Eutypa dieback</Keyword><Keyword MajorTopicYN="N">Pseudomonas poae</Keyword><Keyword MajorTopicYN="N">biocontrol</Keyword><Keyword MajorTopicYN="N">endophyte</Keyword><Keyword MajorTopicYN="N">microbiome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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last="Niem">Jennifer Millera Niem</name><name sortKey="Savocchia, Sandra" sort="Savocchia, Sandra" uniqKey="Savocchia S" first="Sandra" last="Savocchia">Sandra Savocchia</name><name sortKey="Savocchia, Sandra" sort="Savocchia, Sandra" uniqKey="Savocchia S" first="Sandra" last="Savocchia">Sandra Savocchia</name><name sortKey="Stodart, Benjamin" sort="Stodart, Benjamin" uniqKey="Stodart B" first="Benjamin" last="Stodart">Benjamin Stodart</name><name sortKey="Stodart, Benjamin" sort="Stodart, Benjamin" uniqKey="Stodart B" first="Benjamin" last="Stodart">Benjamin Stodart</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GrapevineDiseaseV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000078 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000078 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GrapevineDiseaseV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32273871
|texte= Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32273871" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrapevineDiseaseV1
| This area was generated with Dilib version V0.6.37. |  |